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Functional genomics — through the overexpression, repression, deletion, or conversion of DNA — can reveal much about how genetic alterations lead to changes in phenotype.

The GPP collaborates with researchers in the Ó³»­´«Ã½ community and from academic and industrial partners to apply functional genomics to biological discovery with both precision and scale. This includes Ó³»­´«Ã½ flagship projects, such as the , and investigator-driven projects. 

Through these interactions, the GPP gains critical insight into the strengths and limitations of existing approaches to functional genomics technologies, informing the further development of experimental tools.

Research in the GPP

The works to develop and expand functional genomics technologies for altering gene expression, producing sequence variants, or perturbing gene regulation, selectively or for genome-wide screening. These technologies include:

• CRISPR-Cas9 and/or -Cas12a editing
• transcriptional activation (CRISPRa)
• transcriptional repression (CRISPRi) 
• base editing
• prime editing
• open reading frame (ORF) overexpression
• RNA interference

This work is largely enabled by the Functional Genomics Consortium, a collaborative effort with world-leading life sciences organizations. Knowledge and materials generated through this effort are shared throughout the entire scientific community through publications, reagents available through distributors such as , and our .

Additionally, GPP's team of software engineers designs and maintains many critical tools that support scientists worldwide, including:

• (a portal for designing CRISPR reagents)
• (a base editor tiling library design tool)
• (a tool for modular vector design)