Development of Light-Activated CRISPR Using Guide RNAs with Photocleavable Protectors.
Angew Chem Int Ed Engl
| Authors | |
| Abstract | The ability to remotely trigger CRISPR/Cas9 activity would enable new strategies to study cellular events with greater precision and complexity. In this work, we have developed a method to photocage the activity of the guide RNA called "CRISPR-plus" (CRISPR-precise light-mediated unveiling of sgRNAs). The photoactivation capability of our CRISPR-plus method is compatible with the simultaneous targeting of multiple DNA sequences and supports numerous modifications that can enable guide RNA labeling for use in imaging and mechanistic investigations. |
| Year of Publication | 2016
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| Journal | Angew Chem Int Ed Engl
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| Volume | 55
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| Issue | 40
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| Pages | 12440-4
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| Date Published | 2016 Sep 26
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| ISSN | 1521-3773
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| DOI | 10.1002/anie.201606123
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| PubMed ID | 27554600
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| Links | |
| Grant list | DP1 CA174420 / CA / NCI NIH HHS / United States
P30 CA014051 / CA / NCI NIH HHS / United States
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