Virtual microfluidics for digital quantification and single-cell sequencing.
Nat Methods
| Authors | |
| Abstract | We have developed hydrogel-based virtual microfluidics as a simple and robust alternative to complex engineered microfluidic systems for the compartmentalization of nucleic acid amplification reactions. We applied in-gel digital multiple displacement amplification (dMDA) to purified DNA templates, cultured bacterial cells and human microbiome samples in the virtual microfluidics system, and demonstrated whole-genome sequencing of single-cell MDA products with excellent coverage uniformity and markedly reduced chimerism compared with products of liquid MDA reactions. |
| Year of Publication | 2016
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| Journal | Nat Methods
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| Volume | 13
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| Issue | 9
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| Pages | 759-62
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| Date Published | 2016 Sep
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| ISSN | 1548-7105
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| DOI | 10.1038/nmeth.3955
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| PubMed ID | 27479330
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| PubMed Central ID | PMC5007149
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| Links | |
| Grant list | U54 HG003067 / HG / NHGRI NIH HHS / United States
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