CRISPR Screens to Discover Functional Noncoding Elements.

Trends Genet
Authors
Abstract

A major challenge in genomics is to identify functional elements in the noncoding genome. Recently, pooled clustered regularly interspersed palindromic repeat (CRISPR) mutagenesis screens of noncoding regions have emerged as a novel method for finding elements that impact gene expression and phenotype/disease-relevant biological processes. Here we review and compare different approaches for high-throughput dissection of noncoding elements.

Year of Publication
2016
Journal
Trends Genet
Volume
32
Issue
9
Pages
526-9
Date Published
2016 Sep
ISSN
0168-9525
DOI
10.1016/j.tig.2016.06.004
PubMed ID
27423542
PubMed Central ID
PMC4992445
Links
Grant list
F32 DK096822 / DK / NIDDK NIH HHS / United States
R00 HG008171 / HG / NHGRI NIH HHS / United States