Generation of a TLE1 homozygous knockout human embryonic stem cell line using CRISPR-Cas9.
Stem Cell Res
| Authors | |
| Abstract | Here, we generated a biallelic mutation in the TLE1 (Transducin Like Enhancer of Split 1) gene using CRISPR-Cas9 editing in the human embryonic stem cell (hESC) line WA01. The homozygous knockout cell line, TLE1-464-G04, displays loss of TLE1 protein expression while maintaining pluripotency, differentiation potential and genomic integrity. |
| Year of Publication | 2016
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| Journal | Stem Cell Res
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| Volume | 17
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| Issue | 2
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| Pages | 430-432
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| Date Published | 2016 Sep
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| ISSN | 1876-7753
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| DOI | 10.1016/j.scr.2016.09.009
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| PubMed ID | 27879218
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