CRISPR-Based Technologies for the Manipulation of Eukaryotic Genomes.
Cell
| Authors | |
| Abstract | The CRISPR-Cas9 RNA-guided DNA endonuclease has contributed to an explosion of advances in the life sciences that have grown from the ability to edit genomes within living cells. In this Review, we summarize CRISPR-based technologies that enable mammalian genome editing and their various applications. We describe recent developments that extend the generality, DNA specificity, product selectivity, and fundamental capabilities of natural CRISPR systems, and we highlight some of the remarkable advancements in basic research, biotechnology, and therapeutics science that these developments have facilitated. |
| Year of Publication | 2017
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| Journal | Cell
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| Volume | 168
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| Issue | 1-2
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| Pages | 20-36
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| Date Published | 2017 Jan 12
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| ISSN | 1097-4172
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| DOI | 10.1016/j.cell.2016.10.044
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| PubMed ID | 27866654
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| PubMed Central ID | PMC5235943
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| Links | |
| Grant list | R35 GM118062 / GM / NIGMS NIH HHS / United States
Howard Hughes Medical Institute / United States
R01 GM065400 / GM / NIGMS NIH HHS / United States
F32 GM112366 / GM / NIGMS NIH HHS / United States
R01 EB022376 / EB / NIBIB NIH HHS / United States
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