Immobilization of actively thromboresistant assemblies on sterile blood-contacting surfaces.

Adv Healthc Mater

Authors	Zheng Qu Venkat Krishnamurthy Carolyn Haller Brent Dorr Ulla Marzec Sawan Hurst Monica Hinds Stephen Hanson David Liu Elliot Chaikof
Keywords	Animals Disease Models, Animal Bacterial Proteins Amines Staphylococcus aureus Aminoacyltransferases Cysteine Endopeptidases Biotin Papio Coated Materials, Biocompatible Blood Platelets Thrombomodulin Thrombosis Azides Heparin Immobilized Proteins Polyethylene Glycols Polytetrafluoroethylene
Abstract	Rapid one-step modification of thrombomodulin with alkylamine derivatives such as azide, biotin, and PEG is achieved using an evolved sortase (eSrtA) mutant. The feasibility of a point-of-care scheme is demonstrated herein to site-specifically immobilize azido-thrombomodulin on sterilized commercial ePTFE vascular grafts, which exhibit superior thromboresistance compared with commercial heparin-coated grafts in a primate model of acute graft thrombosis.
Year of Publication	2014
Journal	Adv Healthc Mater
Volume	3
Issue	1
Pages	30-5
Date Published	2014 Jan
ISSN	2192-2659
DOI	10.1002/adhm.201300110
PubMed ID	23788402
PubMed Central ID	PMC3955951
Links
Grant list	R01 HL056819 / HL / NHLBI NIH HHS / United States R01 HL083867 / HL / NHLBI NIH HHS / United States R01 HL106018 / HL / NHLBI NIH HHS / United States

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