LC/MS analysis of cellular RNA reveals NAD-linked RNA.
Nat Chem Biol
| Authors | |
| Keywords | |
| Abstract | We developed a general method to detect cellular small molecule-RNA conjugates that does not rely on the reactivity of the small molecule. This technique revealed NAD-linked RNA in Escherichia coli and Streptomyces venezuelae. Subsequent characterization showed that NAD is a 5' modification of RNA, cannot be installed in vitro through aberrant transcriptional initiation, is only found among smaller cellular RNAs and is present at a surprisingly high abundance of approximately 3,000 copies per cell. |
| Year of Publication | 2009
|
| Journal | Nat Chem Biol
|
| Volume | 5
|
| Issue | 12
|
| Pages | 879-81
|
| Date Published | 2009 Dec
|
| ISSN | 1552-4469
|
| DOI | 10.1038/nchembio.235
|
| PubMed ID | 19820715
|
| PubMed Central ID | PMC2842606
|
| Links | |
| Grant list | R01GM065865 / GM / NIGMS NIH HHS / United States
R01 GM065865-05A1 / GM / NIGMS NIH HHS / United States
Howard Hughes Medical Institute / United States
R01 GM065865-06S1 / GM / NIGMS NIH HHS / United States
R01 GM065865-06 / GM / NIGMS NIH HHS / United States
R01 GM065865 / GM / NIGMS NIH HHS / United States
|