Proton and nitrogen sequential assignments and secondary structure determination of the human FK506 and rapamycin binding protein.
Biochemistry
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| Abstract | Sequential 1H and 15N assignments of human FKBP, a cytosolic binding protein for the immunosuppressive agents FK506 and rapamycin, are reported. A combination of homonuclear and relayed heteronuclear experiments has enabled assignment of 98 of 99 backbone amide NHs, 119 of 120 C alpha Hs, 97 of 99 non-proline amide 15Ns, and 375 of 412 side-chain resonances of this 107-residue protein. Long-range NOEs are used to demonstrate that FKBP has a novel folding topology consisting of a five-stranded antiparallel beta sheet with +3, +1, -3, +1 loop connectivity. |
| Year of Publication | 1991
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| Journal | Biochemistry
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| Volume | 30
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| Issue | 19
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| Pages | 4774-89
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| Date Published | 1991 May 14
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| ISSN | 0006-2960
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| PubMed ID | 1709363
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| Grant list | 1-S10-RR04870 / RR / NCRR NIH HHS / United States
GM-30804 / GM / NIGMS NIH HHS / United States
GM-38627 / GM / NIGMS NIH HHS / United States
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