Discovery of proteins associated with a predefined genomic locus via dCas9-APEX-mediated proximity labeling.

Nat Methods

Authors	Samuel Myers Jason Wright Ryan Peckner Brian Kalish Feng Zhang Steven Carr
Keywords	Humans Chromatin Immunoprecipitation Genome Genomics Polymerase Chain Reaction HEK293 Cells Endonucleases Protein Engineering DNA-(Apurinic or Apyrimidinic Site) Lyase CRISPR-Associated Protein 9 Multifunctional Enzymes
Abstract	Regulation of gene expression is primarily controlled by changes in the proteins that occupy genes' regulatory elements. We developed genomic locus proteomics (GLoPro), in which we combine CRISPR-based genome targeting, proximity labeling, and quantitative proteomics to discover proteins associated with a specific genomic locus in native cellular contexts.
Year of Publication	2018
Journal	Nat Methods
Volume	15
Issue	6
Pages	437-439
Date Published	2018 06
ISSN	1548-7105
DOI	10.1038/s41592-018-0007-1
PubMed ID	29735997
PubMed Central ID	PMC6202184
Links
Grant list	U24 CA160034 / CA / NCI NIH HHS / United States R01 MH110049 / MH / NIMH NIH HHS / United States U24 CA210986 / CA / NCI NIH HHS / United States R01 HG009761 / HG / NHGRI NIH HHS / United States U24 CA126476 / CA / NCI NIH HHS / United States RM1 HG006193 / HG / NHGRI NIH HHS / United States