A scalable, fully automated process for construction of sequence-ready barcoded libraries for 454.

Genome Biol

Authors	Niall Lennon Robert Lintner Scott Anderson Pablo Alvarez Andrew Barry William Brockman Riza Daza Rachel Erlich Georgia Giannoukos Lisa Green Andrew Hollinger Cindi Hoover David Jaffe Frank Juhn Danielle McCarthy Danielle Perrin Karen Ponchner Taryn Powers Kamran Rizzolo Dana Robbins Elizabeth Ryan Carsten Russ Todd Sparrow John Stalker Scott Steelman Michael Weiand Andrew Zimmer Matthew Henn Chad Nusbaum Robert Nicol
Keywords	Humans Algorithms Sequence Analysis, DNA Gene Library High-Throughput Screening Assays Microspheres Automatic Data Processing
Abstract	We present an automated, high throughput library construction process for 454 technology. Sample handling errors and cross-contamination are minimized via end-to-end barcoding of plasticware, along with molecular DNA barcoding of constructs. Automation-friendly magnetic bead-based size selection and cleanup steps have been devised, eliminating major bottlenecks and significant sources of error. Using this methodology, one technician can create 96 sequence-ready 454 libraries in 2 days, a dramatic improvement over the standard method.
Year of Publication	2010
Journal	Genome Biol
Volume	11
Issue	2
Pages	R15
Date Published	2010
ISSN	1474-760X
URL
DOI	10.1186/gb-2010-11-2-r15
PubMed ID	20137071
PubMed Central ID	PMC2872875
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