Targeted next-generation sequencing of a cancer transcriptome enhances detection of sequence variants and novel fusion transcripts.
Genome Biol
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| Abstract | Targeted RNA-Seq combines next-generation sequencing with capture of sequences from a relevant subset of a transcriptome. When testing by capturing sequences from a tumor cDNA library by hybridization to oligonucleotide probes specific for 467 cancer-related genes, this method showed high selectivity, improved mutation detection enabling discovery of novel chimeric transcripts, and provided RNA expression data. Thus, targeted RNA-Seq produces an enhanced view of the molecular state of a set of "high interest" genes. |
| Year of Publication | 2009
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| Journal | Genome Biol
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| Volume | 10
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| Issue | 10
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| Pages | R115
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| Date Published | 2009
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| ISSN | 1474-760X
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| URL | |
| DOI | 10.1186/gb-2009-10-10-r115
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| PubMed ID | 19835606
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| PubMed Central ID | PMC2784330
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| Grant list | DP2OD002750 / OD / NIH HHS / United States
HG03067-05 / HG / NHGRI NIH HHS / United States
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