High-definition spatial transcriptomics for in situ tissue profiling.
Nat Methods
| Authors | |
| Abstract | Spatial and molecular characteristics determine tissue function, yet high-resolution methods to capture both concurrently are lacking. Here, we developed high-definition spatial transcriptomics, which captures RNA from histological tissue sections on a dense, spatially barcoded bead array. Each experiment recovers several hundred thousand transcript-coupled spatial barcodes at 2-μm resolution, as demonstrated in mouse brain and primary breast cancer. This opens the way to high-resolution spatial analysis of cells and tissues. |
| Year of Publication | 2019
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| Journal | Nat Methods
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| Date Published | 2019 Sep 09
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| ISSN | 1548-7105
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| DOI | 10.1038/s41592-019-0548-y
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| PubMed ID | 31501547
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| Links | |
| Grant list | 2016.0454 / Knut och Alice Wallenbergs Stiftelse (Knut and Alice Wallenberg Foundation)
ALTF 738-2017 / European Molecular Biology Organization (EMBO)
1OT2OD026673-1 / U.S. Department of Health & Human Services | National Institutes of Health (NIH)
P2ZHP3_181475 / Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (Swiss National Science Foundation)
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