Development of a High-Throughput Functional Screen Using Nanowell-Assisted Cell Patterning.

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Authors	Ayca Ozkumur Brittany Goods J Christopher Love
Keywords	Animals Humans Cell Proliferation Cell Movement HIV Infections Drug Design HIV-1 False Positive Reactions High-Throughput Screening Assays Microscopy, Fluorescence Nanoparticles Nanotechnology Biological Products CHO Cells Cricetinae Cricetulus Anti-HIV Agents ROC Curve Microfluidics Antibodies, Neutralizing HIV Antibodies Neutralization Tests
Abstract	Living-cell-based screens can facilitate lead discovery of functional therapeutics of interest. A versatile and scalable method is reported that uses dense arrays of nanowells for imparting defined patterns on monolayers of cells. It is shown that this approach can coordinate a multi-component biological assay by designing and implementing a high-throughput, functional nanoliter-scale neutralization assay to identify neutralizing antibodies against HIV.
Year of Publication	2015
Journal	Small
Volume	11
Issue	36
Pages	4643-50
Date Published	2015 Sep
ISSN	1613-6829
URL
DOI	10.1002/smll.201500674
PubMed ID	26121321
PubMed Central ID	PMC4754792
Links
Grant list	P30 CA014051 / CA / NCI NIH HHS / United States U19 AI090970 / AI / NIAID NIH HHS / United States 5 U19 A1090970 / PHS HHS / United States P30-CA14051 / CA / NCI NIH HHS / United States

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