Ó³»­´«Ã½

SUMMARY: Post-sequencing quality control is a crucial component of RNA sequencing (RNA-seq) data generation and analysis, as sample quality can be affected by sample storage, extraction, and sequencing protocols. RNA-seq is increasingly applied to cohorts ranging from hundreds to tens of thousands of samples in size, but existing tools do not readily scale to these sizes, and were not designed for a wide range of sample types and qualities. Here, we describe RNA-SeQC 2, an efficient reimplementation of RNA-SeQC (DeLuca et al., 2012) that adds multiple metrics designed to characterize sample quality across a wide range of RNA-seq protocols.

AVAILABILITY AND IMPLEMENTATION: The command-line tool, documentation, and C ++ source code are available at the GitHub repository .

SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.