Gain biological insights through image-based profiling using CellProfiler and Morpheus
October 31, 2024, 12-5 pm |
Presenter
Shatavisha Dasgupta, MD, PhD, Postdoctoral Associate, Imaging Platform, Ó³»´«Ã½ of MIT and Harvard
Microscopy images contain a lot more information than what our eyes can perceive. In recent years, automated microscopy has streamlined and simplified the process of acquiring thousands of images of cells that have been subjected to chemical or genetic perturbations. Image-based profiling is the method that allows us to mine the rich data contained in these images, to measure and compare the effects of these perturbations on the cells, and thereby gain new insights into a wide variety of research questions, such as understanding gene functions or mechanisms of action of drugs. This workshop aims to provide an overview of the basic concepts of image analysis, and teach how to use open-source tools such as CellProfiler to perform thousands of measurements for each individual cell, and the web-tool Morpheus to determine the relationships of these sets of measurements (features) with the perturbations.
Agenda
- Check-in and pick-up box lunch - 30 mins
- Introduction to CellProfiler - 20 mins
- Hands-on exercise – Beginner segmentation - 2 hrs
- Q and A / Break - 20 mins
- Introduction to morphological profiling - 20 mins
- Hand-on exercise – Profiling with Morpheus - 1hr
