SNP genotyping using the Sequenom MassARRAY iPLEX platform.
Curr Protoc Hum Genet
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| Abstract | The method for SNP genotyping described in this unit is based on the commercially available Sequenom MassARRAY platform. The assay consists of an initial locus-specific PCR reaction, followed by single base extension using mass-modified dideoxynucleotide terminators of an oligonucleotide primer which anneals immediately upstream of the polymorphic site of interest. Using MALDI-TOF mass spectrometry, the distinct mass of the extended primer identifies the SNP allele. |
| Year of Publication | 2009
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| Journal | Curr Protoc Hum Genet
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| Volume | Chapter 2
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| Pages | Unit 2.12
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| Date Published | 2009 Jan
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| ISSN | 1934-8258
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| URL | |
| DOI | 10.1002/0471142905.hg0212s60
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| PubMed ID | 19170031
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