Cas9 gRNA engineering for genome editing, activation and repression.
Nat Methods
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| Abstract | We demonstrate that by altering the length of Cas9-associated guide RNA (gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits with combined transcriptional regulation and kill functions governed by a single multifunctional Cas9 protein. |
| Year of Publication | 2015
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| Journal | Nat Methods
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| Volume | 12
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| Issue | 11
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| Pages | 1051-4
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| Date Published | 2015 Nov
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| ISSN | 1548-7105
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| URL | |
| DOI | 10.1038/nmeth.3580
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| PubMed ID | 26344044
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| PubMed Central ID | PMC4666719
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| Grant list | P50 HG005550 / HG / NHGRI NIH HHS / United States
R01 CA155320 / CA / NCI NIH HHS / United States
T32 GM007598 / GM / NIGMS NIH HHS / United States
P50 GM098792 / GM / NIGMS NIH HHS / United States
Canadian Institutes of Health Research / Canada
T32 CA009216 / CA / NCI NIH HHS / United States
5T32CA009216-34 / CA / NCI NIH HHS / United States
5R01CA155320-04 / CA / NCI NIH HHS / United States
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